© 2019 Lee Harris Photography, all rights reserved.
EDUCATION
University of Barcelona. Faculty of Biology. Degree in Biology (1983-1988).
University of Barcelona. PhD in Biological Sciences (1994-1998) Department of Physiology and Immunology. “Phenotypic and functional activity of P-Glycoprotein: Implications for multidrug resistance in cancer”.
PROFESSIONAL TRAINING
Feb 2022- present: Project Leader Scientist. Germasn Trias i Pujol Research Institute. Badalona, Barcelona, Spain.
Jan 2015- Dec 2021: Project Leader Scientist. Josep Carreras Leukemia Research Institute. Badalona, Barcelona, Spain.
Feb 2006- Jul 2014: Project Leader Scientist. Institut de Recerca. Hospitall Vall d’Hebron, Barcelona, Spain.
Sep 2000-Jan 2006: Project Leader Scientist (Ramon y Cajal Program). IDIBAPS, Barcelona, Spain.
Oct 1997-Sep 1999: Research Associate. Institut Recerca Oncologica (IRO), Barcelona, Spain. (Substitution).
Sep 1991-Sep 1997: Graduate student. Institut de Recerca Oncologica (IRO), Barcelona, Spain.
Jul 1985-Sep 1991: Scientific collaborator. The Animal Biology Department. University of Barcelona. Barcelona, Spain.
FELLOWSHIPS
- Postdoctoral Fellowship IRO. (Oct 1999-Mar 2000).
- Predoctoral Fellowship IRO. (Sep 1991-Sep1997).
TECHNICAL EXPERIENCE
-
Culture of eukaryotic cell lines. Cloning and selection of specific
cell types. Gene transfer to eukaryotic cell techniques:
electroporation, calcium phosphate precipitation,
lipofection.
- Flow cytometry and cell sorting: multicolor fluorescence analysis
(14-colors), combined intracellular and surface staining, analysis
of rare cells (CD34 and Side Population), high resolution analysis
of DNA content, programmed cell death assessment, simultaneous
detection of BrdU, EDU, incorporation and DNA staining, analysis of
intracellular calcium, functional analysis of drug resistant cells,
Flow-FISH.
- Basic techniques in molecular biology.
- Gas chromatography analysis.
- Scanning electron microscopy.
PATENTS
-
Petriz J. Assemblable cell filtering unit. Patent #P200401163.
Institution: IDIBAPS.
COMMITTEES,
PROFESSIONAL SOCIETIES AND REVIEW GROUPS
-
International Society for Clinical Cytometry Outreach Committee
Member (2020-present)
- Clinical Cytometry Education Network (CCEN) Course Committee
Member (2017-19)
- Membership & Administration Committee, ESCCA (2018-19)
- Councilor at European Society for Clinical Cell Analysis (ESCCA)
Executive Board (2015-18)
- President of the Iberian Society for Cytometry (2007-2015)
- Reviewer of Cytometry. The official journal of the International
Society for Advancement of Cytometry (ISAC)
- Member of the International Society for Advancement of
Cytometry
- Member of the European Society for Clinical Cell Analysis
(ESCCA)
-
Member of the Subcommittee on Quality Assessment of Hematopietic
Stem Cell Grafts (EBMT, European Group for Bone Marrow
Transplantation)
INVITED
PRESENTATIONS AT INTERNATIONAL CONFERENCES
- PD-L1 in Myeloid Derived Suppressor Cells (MDSCs)
Primers 2022, Nov 25, IGTP, Badalona (Spain)
- Minimal sample perturbation assays for cell immnophenotyping and
functional analysis
AFC 2022, Nov 21, Nice (France)
- Cytometry Data Presentation for Quality Publishing
Ciemat, Nov 18, Madrid (Spain)
- Functional Cytomics in the Study of Stem Cells and Leukemia /
Research Integrity and Data Interpretation
Máster Universitario en Aproximaciones Moleculares en Ciencias de
la Salud, Valencia, Nov 11 (Spain)
- PD-L1 expression in Myeloid-Derived Suppressor Cells as a
potential biomarker to determine treatment response to cancer
immunotherapy
CSI, Oct 25, Dublin (Ireland)
- Flow Virometry for Detection of SARS-CoV-2 Particles /
Fast-screening flow cytometry method for detecting nanoplastics in
human peripheral blood
Research Integrity and Data Interpretation
UNEX 2022, Sep 26, Badajoz (Spain)
- Cytometry Data Presentation for Quality Publishing
ESCCA 2022, Sep 24, Belfast, North Ireland (UK)
XXII Curso de Fundamentos y Aplicaciones en Citometría de Flujo,
IGTP, June 16, 2022, Badalona (Spain)
- Fast-screening flow cytometry method for detecting nanoplastics
in human peripheral blood
Romanian Cytometry Meeting, June 14, 2022 Bucarest (Romania)
- Stem Cell Biology Methods and Practice
Master in Translational Medicine, March 9, 2022 Barcelona
(Spain)
- Multidrug Resistance
Master's Degree in Molecular Approaches in Health Sciences, March
8, 2022 Valencia (Spain)
- Flow Virometry for Detection of SARS-CoV-2 Particles. Romanian
Cytometry Meeting, Virtual Interactive, June 14, 2022
- Detection of SARS-CoV-2 using Flow Virometry. Science against
SARS-CoV-2: Achievements & Advancements. Virtual Interactive
June 23, 2021.
- A rapid flow virometric test for detection of SARS-CoV-2
particles.
The International Society for Advancement of Cytometry. Virtual
Interactive June 7-19, CYTO 2021 (Virtual oral presentation).
- A rapid flow virometric test for detection of SARS-CoV-2
particles. The Iberian Society for Cytometry. Virtual Interactive
June 14-10 SIC 2021
- Flow virometry for detection of SARS-CoV-2 particles. NxT User
Meeting. Virtual Interactive May 25, 2021
- Analytical challenges of SARS-CoV-2 detection using flow
virometry. Nano-Cytometry Course. Badajoz, Sep28, 2021.
- The Role of Acoustic Cytometry in Cancer Immunotherapy and
Leukemia Research. March 20. London (2019)
- 什麼是NxT以及如何使隱形看得見.
Octubre 4, Taipei, Taiwan (2018)
- Flow Oddities: Take Your Fluorescent Probes and Put The Acoustics
On. September 17, Modena, Italia (2018)
- Good Cytometry Practices in Cell Proliferation and Cell Death.
September 14, ESCCA, Valencia, Spain (2018)
- Myths and Realities about Microvesicle Analysis. Spetember 12,
ESCCA, Valencia, Spain (2018)
- What’s NxT and How to Make Visible the Invisible. CYTO Prague
(2018)
-
The Science behind CD34 counting. 6th CCEN Course, Clearwater,
Florida, USA (2018)
- Differential expression of PD-L1 on Myeloid Derived Suppressor
Cells (MDSCs). 6th CCEN Course, Clearwater, Florida, USA
(2018)
- Cancer stem cells and mechanisms of multidrug resistance by flow
cytometry. CYTOAsia, Singapore (2017)
- Cancer stem cells and mechanisms of multidrug resistance by flow
cytometry. ESCCA, Thessaloniki, Greece (2017)
- Past present and Future of Hematopoietic Stem Cell Counting. 7th
European Scientific Symposium, Ghent, Belgium (2017)
- Next generation flow cytometry for CD34+ progenitor cell
counting. Summer School on Cytometry. Valencia, Spain (2017)
- Use of flow cytometry and alkaline phosphatase as a phenotypic
marker of PSCs and primitive stem cells. Stem Cell Day, British
Museum, London (2016)
- Flow cytometry no-lyse no-wash approach for rare event detection
and cytotoxicity analysis. Regulatory T Cell Symposium. Leuven,
Belgium (2016)
- Cancer stem cells and mechanisms of multidrug resistance by flow
cytometry. CYToMORE, Modena, Italy (2016)
- Cancer stem cells and mechanisms of multidrug resistance by flow
cytometry. Molecular Probes Flow Cytometry Webinars (2016)
- Functional heterogeneity of CD34 positive and CD34 negative
cells. Paul Ehrlich Lecture Hall, Charité Campus Mitte, Berlin
(2015)
- Stem cells ans ABC multidrug transporters. Paul Ehrlich Lecture
Hall, Charité Campus Mitte, Berlin (2015)
- In search of hematopoietic bone marrow stem cells: Functional
heterogeneity of CD34+ and CD34-negative cells. SIC Meeting. Las
Palmas de Gran Canaria (2015).
- Alkaline phosphatase live stain for stem cell research. Meeting
of the Sociedade Ibérica de Citometria. Aveiro, Portugal
(2013).
- New Horizons in Clinical Cytometry. Workshop Intercalar de la
Sociedad Ibérica de Citometría. Valencia, Spain (2012).
- Characterization of a Side Population of Cancer Cells from Human
Brain Tumors. 10th Euroconference on Clinical Cell Analysis.
Valencia, Spain (2010).
- Side Population analysis for the identification of Stem Cells.
5th Advanced Course in Flow Cytometry. Modena, Italy
(2009).
-
Stem cells by Cytomics. ESUR Meeting. Barcelona, Spain
(2008).
- Side Population and Multidrug Resistance. ELSUG Meeting.
Edinburgh, UK (2007).
- Polychromatic Flow Cytometry and Color Compensation. 10th Meeting
of the Sociedad Ibérica de Citometria. Barcelona, Spain
(2007).
- Side Population stem cells. Advances in Cell Therapy. Madrid,
Spain (2006).
- Flow Cytometry of the Stem Cell Side Population: Tips and tricks.
New Frontiers in Flow Cytometry. Moscow, Russia (2005).
- Flow cytometry of the Side Population. ELSUG Meeting. Barcelona,
Spain (2005).
- New Cytomic Trends in Immuno-Hematology. International Workshop:
The Cell Within Cytomics. Cáceres, Spain (2004).
- Flow cytometry counting of CD34 cells in whole blood. 8th Meeting
of the Sociedad Ibérica de Citometria. Madrid, Spain (2003).
- Analysis of DNA probes by flow cytometry. 8th Meeting of the
Sociedad Ibérica de Citometria. Madrid, Spain (2003).
- Clinical Flow cytometry for multifrug resistance analysis.
Cytometry Applications in Cellular Biology and Medicine. Braga,
Portugal (2003).
- Clinical Applications of Cell Sorting. Cytometry Applications in
Cellular Biology and Medicine. Braga, Portugal (2003).
- Flow Cytometry Analysis of Stem Cells. 43rd International Meeting
of the European Tissue Culture Society. Granada, Spain
(2001).
- Oxidative burst assessment in whole blood leukocytes. 7th Meeting
of the Sociedade Ibérica de Citometria. Coimbra, Portugal
(2001).